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Invitrogen™ Image-iT™ TMRM Reagent (mitochondrial membrane potential indicator)

Product Code. 15656139
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5 x 100 μL
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Product Code. 15656139 Supplier Invitrogen™ Supplier No. I34361

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Description

Healthy mitochondrial membranes maintain a difference in electrical potential between the interior and exterior of the organelle, referred to as a membrane potential.

Tetramethylrhodamine, methyl ester (TMRM) is a cell-permeant dye that accumulates in active mitochondria with intact membrane potentials. If the cells are healthy and have functioning mitochondria, the signal will be bright. Upon loss of the mitochondrial membrane potential, TMRM accumulation will cease and the signal will dim or disappear. TMRM signal can be detected with fluorescence microscopy, flow cytometry, cell sorting, high-throughput screening, and high content analysis.

• For live-cell dynamic study of mitochondrial membrane potential (not fixable)
• Detectable with RFP/TRITC filter set (absorbance peak: 548 nm, emission peak: 574 nm)
• multiplexable with green, deep red, violet, and blue emission dyes, such as GFP, DAPI, Hoechst, HCS LipidTOX, and LysoTracker Deep Red

TRUSTED_SUSTAINABILITY

Specifications

Color Orange
Content And Storage Store in freezer -5°C to -30°C and protect from light.
Product Type Reagent
Product Line Molecular Probes
Quantity 5 x 100 μL
Shipping Condition Wet Ice

Frequently Asked Questions (FAQs)

For a final staining solution, we can add TMRM directly into cell growth medium. Can this media contain serum?

Yes, you may add TMRM to media with serum. Serum components may bind dye non-specifically so you may need a higher final working concentration for staining samples in media containing serum relative to staining the same cells in a non-serum media or buffer.
How is TMRM different from the MitoTracker dyes?

TMRM is a dynamic mitochondrial stain. There is no covalent attachment of TMRM to the mitochondria and because of this, it can move in and out of the mitochondria dependent upon changes in the membrane potential. The MitoTracker dyes on the other hand possess a reactive chloromethyl group that allows for their covalent attachment to mitochondria. As a result, once they accumulate within mitochondria that have a normal membrane potential, they are retained.

What is included in the Image-iT TMRM Reagent (Cat No. I34361)?

The Image-iT TMRM Reagent is a ready-to-use 1,000X concentrated stock solution of the TMRM dye. It contains 5 vials of 100 µM of TMRM in neat DMSO, with 100 µL volume in each vial. TMRM powder (Cat No. T668) is not included in this kit.

For Research Use Only. Not for use in diagnostic procedures.

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