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Novus Biologicals™ INSTA-Blot Human Tissues

Product Code. 18736423 Shop All Bio Techne Products
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1 Membrane
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18736423 1 Membrane Each
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Product Code. 18736423 Supplier Novus Biologicals™ Supplier No. NBP230113

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Description

Applications: Western Blot

  • Screening proteins from various human tissues
  • Contains 11 human tissue lysates (20μg total protein per lane) on 4-20% Tris-Glycine mini gel
  • Prepared from resolution by SDS-PAGE at 125V for 1 hour
  • Proteins are electro-blotted for 2 hours at 25V onto PVDF membrane
  • Stained with amido black for visualization of proteins

Recommended Storage

Store at room temperature

TRUSTED_SUSTAINABILITY

Specifications

For Use With (Application) Tissues are from donors with no known disease. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol). Use in western blot reported in scientific literature (PMID: 24704276) .
Surface Western Blot
Quantity 1 Membrane
Material PVDF
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