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Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer Set
Designed for use in intracellular staining and flow cytometric analysis and has been specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and increase fluorescence signal-to-noise ratios.
Brand: Invitrogen™ 88-8824-00
Additional Details : Weight : 0.00100kg
Alert:
Contains Sodium azide and formaldehyde
Includes: IC Fixation Buffer
Permeabilization Buffer (10X)
Description
- In the first step, live cells can be 'fixed' with the Fixation Buffer, which cross- links proteins
- The second step, using the Permeabilization Buffer, creates holes in the membrane and allows the intracellular staining antibodies to enter the cell effectively
- Subsequent washing steps, antibody additions, and incubations after cell permeabilization should be performed using the Permeabilization Buffer
- Final washing step and cell resuspension prior to running the sample is done using Flow Cytometry Staining Buffer (cat. 00-4222).
- This kit is commonly used to stain cytoplasmic proteins and proteins in the secretory pathway such as chemokines and cytokines
- Not recommended for intranuclear staining. Instead, please refer to the Foxp3/Transcription Factor Buffer Set for nuclear staining
- Before the fixation step, it is recommended to label the live cells using one of the Fixable Viability Dyes and then proceed with fixation
Specifications
| eBioscience™ | |
| Fixation/Permeabilization Buffer Set | |
| Fixation/Permeabilization Buffer |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- eBioscience Intracellular Fixation & Permeabilization Buffer Set
Signal Word
- Danger
Hazard Category
- Carcinogenicity Category 1A
- Serious eye damage/eye irritation Category 1
- Germ cell mutagenicity Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity Category 2
Hazard Statement
- H317-May cause an allergic skin reaction.
- H318-Causes serious eye damage.
- H341-Suspected of causing genetic defects.
- H350-May cause cancer.
- H371-May cause damage to organs.
Precautionary Statement
- P201-Obtain special instructions before use.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P310-Immediately call a POISON CENTER/doctor/.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Paraformaldehyde
For Research Use Only. Not for use in diagnostic procedures.