Learn More
Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase
AccuPrime™ GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.
Brand: Invitrogen™ 12337024
Additional Details : Weight : 0.03000kg
Description
AccuPrime™ GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).
Benefits of using AccuPrime™ GC-Rich DNA Polymerase:
• Yield—high yields for targets up to 5 kb in length
• Specificity—AccuPrime™ accessory proteins for improved PCR specificity
• Sensitivity—sensitivity down to 5 ng of template DNA
Robust and specific amplifications
The AccuPrime™ buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.
Unit definition
One unit of AccuPrime™ GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min at 74°C.
Specifications
• AccuPrime GC-Rich DNA Polymerase (1 x 500 μL at 2 U/μL) • 5X AccuPrime GC-Rich Buffer A (1 x 5 mL) • 5X AccuPrime GC-Rich Buffer B (1 x 5 mL) • 50 mM MgSO4 (1 x 5 mL) Store at –20°C. |
|
AccuPrime GC-Rich DNA Polymerase | |
Wet or Dry Ice | |
2X | |
1000 Reactions | |
Separate Components | |
500 μL |
High | |
Standard | |
Hot-start PCR | |
Built-In Hot Start | |
Blunt | |
5 kb or less |
For Research Use Only. Not for use in diagnostic procedures.