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Invitrogen™ pEXP4-DEST Vector Kit
Description
Includes
pEXP4-DEST vector, positive expression control plasmid, Tag-On-Demand slyD Mutant E. coli Extract, E. coli Reaction Buffer, Tag-On-Demand Suppressor tRNA, Methionine, DNase/RNase-free distilled water, RNase A, T7 Enzyme Mix, 2mL reaction tubes, Lumio Green Detection Kit, and the Benchmark Fluorescent Protein Standard. The pEXP4-DEST Gateway Vector Kit includes pEXP4-DEST and a control plasmid.
- Quickly screen for expression levels of full-length protein
- Save time and effort by cloning only once to express tagged or native protein
- Express C-terminally tagged protein from Ultimate ORF Clone in in vitro system
- Includes specialized E. colilysate derived from slyD mutant for cell-free synthesis: This lysate eliminates non-specific binding of LumioGreen Detection Reagent to endogenous SlyD protein, providing optimal background for recombinant protein detection
- Clone gene of interest containing TAG (amber) stop codon into provided pEXP4-DEST
- Features attR sites for efficient recombination, C-terminal Lumio and 6 x His tags for simplified identification and purification, and components for cell-free protein synthesis
- In presence of ExpresswayTag-On-Demand™ Suppressor tRNA, the TAG codon is not recognized and translation continues through Lumio and 6 x His tags
- In absence of the Suppressor tRNA, TAG stop codon is recognized and native protein is produced
- From same vector, can rapidly determine expression level of C-terminal-tagged, full-length protein and then express native protein free from any adverse affects tag may have on its structure, activity, or molecular interactions
Cell Free Protein Expression for Functional Analysis, Cell-Free (in vitro) Expression, Cloning, Gateway Cloning, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
Specifications
| Promoter | T7 |
| Product Type | Gateway System Destination Expression Vector |
| Selection Agent (Eukaryotic) | None |
| Content And Storage | The Expressway™ Tag-On-Demand™ Cell-Free E. coli Expression System with Lumio™ Technology includes pEXP4-DEST vector (when indicated), positive expression control plasmid, Tag-On-Demand™ slyD Mutant E. coli Extract, E. coli Reaction Buffer, Tag-On-Demand™ Suppressor tRNA, Methionine, DNase/RNase-free distilled water, RNase A, T7 Enzyme Mix, 2-ml reaction tubes, Lumio™ Green Detection Kit, and the Benchmark™ Fluorescent Protein Standard. The pEXP4-DEST Gateway™ Vector Kit includes pEXP4-DEST and a control plasmid. Store vectors, detection reagent, and protein standard at -20°C. Store all other components at -80°C. Guaranteed stable for 6 months when properly stored. |
| Antibiotic Resistance Bacterial | Ampicillin (AmpR), Zeocin™ (ZeoR) |
| Protein Tag | Lumio |
| Cloning Method | Gateway |
| Quantity | 6 μg |
| Vector | pEXP, pDEST |
| Product Line | Expressway, Gateway |
For Research Use Only. Not for use in diagnostic procedures.
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