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Invitrogen™ Taq DNA Polymerase, recombinant
A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer
Marke: Invitrogen™ 10342178
Weitere Details : Weight : 0.11000kg
Beschreibung
The enzyme consists of a single polypeptide with a molecular weight of 94kDa. It has a 5'-3' DNA polymerase activity and a 5'-3' exonuclease activity.
- Choice of recombinant or native enzyme
- Amplification of PCR products up to 5kb in size
- An enzyme that is licensed and qualified for PCR
- Source: Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli
- Applications: Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing
- Unit definition: One unit of TaqDNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C
Spezifikation
| Contains: • 1000 μl Taq DNA Polymerase (5 U/μl) • 20 ml 10X PCR buffer (200 mM Tris-HCl pH 8.4, 500 mM KCl) • 10 ml Magnesium Chloride (50 mM) Store at -30°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly. |
|
| Stand-alone enzyme | |
| qPCR, Standard PCR | |
| Standard | |
| DNA Polymerase | |
| Approved for shipment on Wet or Dry Ice | |
| 1 X | |
| 5000 Reactions | |
| Separate Components | |
| DNA |
| Primer-probe | |
| Low | |
| Taq DNA Polymerase | |
| 5' - 3' | |
| 5000 units | |
| 5 U/μL | |
| No | |
| 3'-A | |
| 5 kb or less |
For Research Use Only. Not for use in diagnostic procedures.