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Invitrogen™ Platinum™ PCR SuperMix High Fidelity

Platinum™ PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.

€ 538.00 - € 16070.00

Specifications

GC-Rich PCR Performance Low
Polymerase Platinum Taq DNA Polymerase High Fidelity
Reaction Speed Standard
Shipping Condition Wet or Dry Ice
For Use With (Application) Hot-start PCR, High-fidelity PCR
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Includes: Platinum PCR SuperMix High Fidelity is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50μL each are provided (4 x 1.125mL). Platinum PCR SuperMix High Fidelity Components: 22U/mL complexed recombinant Taq DNA polymerase, Pyrococcus species GB-D thermostable polymerase, and Platinum Taq Antibody; 66mM Tris-SO4 (pH 8.9); 19.8mM (NH4)2SO4; 2.4mM MgSO4; 220μM dNTPs; and stabilizers.

Products 2
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
10545013
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Invitrogen™
12532016
100 Reactions
€ 538.00
100 reactions
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10175622
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Invitrogen™
12532024
5000 Reactions
€ 16070.00
5000 reactions
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Estimated Shipment: 20-06-2024
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Description

Description

Platinum™ PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. You need only add template and primers, reducing set-up time by half (see figure).

With Platinum™ PCR SuperMix, High Fidelity, you will:
• Achieve greater than six-times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 15 kb
• Produce products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs
• Minimize PCR optimization

Using Platinum™ PCR SuperMix, High Fidelity
Platinum™ PCR SuperMix, High Fidelity, is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic 'hot-start' PCR. This hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

Specifications

Low
Standard
Hot-start PCR, High-fidelity PCR
6X
Mixed
15 kb or less
Platinum Taq DNA Polymerase High Fidelity
Wet or Dry Ice
1.1X
Built-In Hot Start
SuperMix or Master Mix
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.