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Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.

Brand:  Invitrogen™ 12574026

Additional Details : Weight : 0.04454kg

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Product Code. 10082922

  • € 1046.00 / 100 reactions
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Description

Description

The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR. Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers and target RNAs from either total RNA or mRNA. The system uses a mixture of SuperScript III Reverse Transcriptase and Platinum Taq DNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5 kb. The amount of starting material can range from 0.01 pg to 1 μg of total RNA.

Key advantages of SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase
• Sensitive detection down to 0.01 pg total RNA
• Convenient one-step format for increased speed, convenience, and less reaction-to-reaction variability
• SuperScript III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers
• Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility

SuperScript III Reverse Transcriptase
SuperScript III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA.

Platinum Taq DNA Polymerase
Platinum Taq DNA Polymerase is recombinant Taq DNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' in PCR for increased sensitivity, specificity, and yield.

Note: This kit has been optimized for end-point RT-PCR. For RT–qPCR in this product line, use the SuperScript III Platinum One–Step Quantitative RT-PCR System.

Specifications

Specifications

• SuperScript III RT/Platinum Taq Mix (200 μL)
• 2X Reaction Mix (3 x 1 mL)
• 5 mM Magnesium Sulfate (500 μL)

Store at –10 to –30°C.

One-Step RT-PCR System
1-step RT-PCR
30 min.
50°C
Reduced
PCR Amplified cDNA
100 Reactions
Reverse Transcription
RNA
Gel Electrophoresis
High
Platinum Taq DNA Polymerase
1-Step RT-PCR
SuperScript III
Dry Ice
Built-In Hot Start
Premixed Components
Up to 4.5 kb
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For Research Use Only. Not for use in diagnostic procedures.