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Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit

No organic solvents, ethidium bromide, or cesium chloride

Brand:  Invitrogen™ K210004

148.20 EUR valid until 2023-12-01
Use promo code "21275" to get your promotional price.

Additional Details : CAS : 9048-46-8 Weight : 2.00000kg

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Product Code. 10083762

  • € 228.00 / 1191mL
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The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Midiprep Kit protocol will typically yield 100–350µg plasmid DNA from 15–25mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.

  • Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
  • High-purity, low-endotoxin product- pure enough for mammalian cell transfection
  • Versatility- purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs
Anion-exchange chromatography for plasmid purification
  • PureLink HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to level equivalent to two passes through CsCl gradients
  • The resin combines excellent capacity with fast flow rate, high resolution, high yield, and efficient endotoxin removal
  • Plasmid preparations can typically be completed in less than 2 hours
Contaminant-free DNA
  • Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
  • Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of greater than 1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
  • Endotoxin levels are typically within 0.1–1 EU/µg, making this plasmid DNA ideal for mammalian cell transfection
  • Purification time is two hours

Amplified Fragment Length Polymorphism-AFLP™, Bacterial Artificial Chromosome (BAC) Fingerprinting, DNA and RNA Purification and Analysis, DNA Extraction, DNA Sequencing, Fragment Analysis, Midiprep, Plasmid DNA Purification, Sequencing



• 100 ml Resuspension Buffer (R3)
• 550 µl RNase A
• 100 ml Lysis Buffer (L7)
• 100 ml Precipitation Buffer (N3)
• 250 ml Equilibration Buffer (EQ1)
• 500 ml Wash Buffer (W8)
• 125 ml Elution Buffer (E4)
• 15 ml TE Buffer
• 25 HiPure Columns
• 5 Column Holders

Store all components at room temperature.
Anion Exchange Resin
5 ml
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
  • 100mL Resuspension Buffer (R3)
  • 550 μl RNase A
  • 100mL Lysis Buffer (L7)
  • 100mL Precipitation Buffer (N3)
  • 250mL Equilibration Buffer (EQ1)
  • 500mL Wash Buffer (W8)
  • 125mL Elution Buffer (E4)
  • 15mL TE Buffer
  • 25 HiPure Columns
  • 5 Column Holders
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC
25 Preps
Room Temperature
350 µg
Bacterial Culture
BAC DNA, Plasmid DNA
Not High-throughput Compatible (Manual)
25 Preps
100-200 µg (Medium-Scale) Plasmid DNA
Plasmid MidiPrep Kit
Transfection Grade
BAC DNA, Plasmid DNA
2 hrs


Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.