missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Bioactive Small Molecules
  5. 31921410

Tocris Bioscience™ Janelia Fluor™ 552, Haloalkane

Product Code. 31921410
Change view
Click to view available options
Quantity:
10 μg
Unit Size:
10µg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
31921410 10 μg 10µg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 31921410 Supplier Tocris Bioscience™ Supplier No. 8948/10U

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Cell-permeable fluorogenic dye with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag substrate. Also chemigenetic Ca2+ indicator.

Key Information: Janelia Fluor? 552, Haloalkane is a yellow cell-permeable fluorogenic dye with a chloroalkane handle. Also chemigenetic calcium indicator for imaging Ca2+ mediated responses. Upon self-labeling in WHaloCaMP modular biosensors, fluorescence output is modulated by photoinduced electron transfer (PET) to a protein based quencher.

Application: live-cell imaging as a self-labeling tag substrate. Suitable for confocal microscopy, light sheet microscropy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). In vivo functional imaging of Ca2+ responses (flies/mice); multiplexed functional imaging of neurons and astrocytes in zebrafish larvae; quantification of Ca2+ concentration using fluorescence lifetime imaging microscopy (FLIM).

Properties and Photophysical Data: Stable to fixation. Cell permeable. KD = 87 ? 5. In the presence of EGTA (Ca2+ chelator) excitation and emission maxima (λ) are 561 nm and 559-578 nm, respectively; quantum yield = 0.05; extinction coefficient = 73,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); brigthness = 3.6 mM-1cm-1. In the presence of saturating Ca2+, excitation and emission maxima (λ) are 562 nm and 565-582 nm, respectively; quantum yield = 0.25; extinction coefficient = 78,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); brigthness = 19.5 mM-1cm-1.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

TRUSTED_SUSTAINABILITY

Specifications

Chemical Name or Material 2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-((2-(2-((6-chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)benzoate
CAS 2634129-34-1
Quantity 10 μg
Target Self-labeling Tags
Molecular Formula C37H40ClF2N3O6
Purity 90%
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.