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Tocris Bioscience™ Janelia Fluor 646, free acid

Product Code. 18798746
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Product Code. 18798746 Supplier Tocris Bioscience™ Supplier No. 6993/5

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Description

Red dye (Reactive group: free acid). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, flow and live cell imaging

Janelia Fluor® 646, free acid is a red fluorogenic fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor™ HaloTag™ and SNAP-Tag™ ligands, for use in live cell imaging experiments. Suitable for confocal fluorescent imaging, super resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 646 nm; emission maximum = 664 nm; Quantum yield = 0.54; Extinction coefficient = 152,000 M-1cm-1.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor™ conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

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Specifications

Chemical Name or Material 1-[7-(1-Azetidinyl)-10-(2,5-dicarboxyphenyl)-9,9-dimethyl-9-silaanthracen-2(9 H)-ylidene]azetidinium inner salt trifluoroacetate
Quantity 5 mg
Target Free Acid Janelia Fluor™ Dyes
Molecular Formula C29H28N2O4Si.CF3CO2H
Formula Weight 3737
Purity 95%
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