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Ki-67, eFluor 570, clone: 20Raj1, eBioscience™

Mouse Monoclonal Antibody

Merk:  Affymetrix eBioscience 41-5699-80

Overige gegevens : Gewicht : 0.25000kg

 Bekijk meerdere types van dit product via de

Produktcode. 15361070

  • € 99.25 / 25 µg


Geschatte verzenddatum
bij leverancier 03-04-2018
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Beschrijving

Beschrijving

Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist, a 345 and 395kDa form that are expressed in dividing cells. Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells.This monoclonal antibody 20Raj1 recognizes canine Ki-67.Applications Reported: This 20Raj1 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This 20Raj1 antibody has been tested by immunocytochemistry on fixed and permeabilized HeLa cells and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
Specificatie

Specificatie

Ki-67
20Raj1
eFluor 570
Conjugated
IgG1, kappa
RUO
Primary
Human
Immunohistochemistry (Frozen)
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Video's

For Research Use Only.