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Thermo Scientific™ LanthaScreen™ TR-FRET RAR gamma Coactivator Assay Kit, goat
Contains Goat Tb-Anti-GST antibody
Brand: Thermo Scientific™ PV4411
Additional Details : Weight : 0.00900kg
Description
- This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15123:
- The LanthaScreen™ TR-FRET Retinoic Acid Receptor (RAR) gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential RAR gamma ligands as agonists of coactivator recruitment.
- The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
- The LanthaScreen™ TR-FRET RAR gamma Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human RAR gamma ligand-binding domain (RAR gamma-LBD) that is tagged with glutathione-S-transferase (GST).
- To assay, RAR gamma-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.
- The LanthaScreen™ TR-FRET RAR gamma Coactivator Assay Kit contains RAR gamma-LBD (GST) protein, fluorescein-labeled PGC1a coactivator peptide, Tb-anti-GST antibody, and buffers.
- Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Specifications
| Tb (Terbium) | |
| Microplate Reader | |
| 384-well Plate | |
| Biochemical coregulator interaction | |
| End Point | |
| RARG, RAR gamma, NR1B3 | |
| Co-Factor Interaction Assay, TR-FRET | |
| TR-FRET RAR Gamma Coactivator Assay Kit |
| Fluorescent | |
| 800 x 20 μL Assays | |
| Store in ultra-cold freezer (-68 to -85°C). | |
| 5916 | |
| Dry Ice | |
| LanthaScreen™ | |
| RAR gamma |
For Research Use Only. Not for use in diagnostic procedures.