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  5. Invitrogen™ Rat LIX-Cell Lysate (CXCL5) ELISA Kit

Invitrogen™ Rat LIX-Cell Lysate (CXCL5) ELISA Kit

Product Code. 18567834
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
Pack of 10
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Product Code. 18567834

Brand: Invitrogen™ ERCXCL5CLX10

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Description

ELISA

The Rat LIX (CXCL5) ELISA quantitates Rt LIX in rat cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Rt LIX. Principle of the method The Rat LIX solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is an inflammatory chemokine that belongs to the CXC chemokine family. This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P97885
Assay Range 8.23 to 6000 pg/mL
Assay Sensitivity 15 pg/ml
Calibration Range 15 pg/mL to 6000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Chemokine & Receptors
Sample Type Cell Lysates
Specificity The antibody pair provided in this kit recognizes rat LIX / CXCL5.
Sufficient For 96 assays
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene Symbol Cxcl5, Cxcl6
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Research Discipline Immunology
Sample Volume Cell Lysate 20 μL
Storage Requirements 2°C to 8°C
Synonym Cxcl5
Target CXCL5
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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