missing translation for 'onlineSavingsMsg'
Learn More

Ly-49G2, PerCP-eFluor™ 710, clone: eBio4D11 (4D11), eBioscience™

Rat Monoclonal Antibody

Merk:  Affymetrix eBioscience 46-5781-82

Overige gegevens : Gewicht : 0.25000kg

 Bekijk meerdere types van dit product via de

Produktcode. 15331350

  • € 300.00 / 100 µg


Geschatte verzenddatum
bij leverancier 27-12-2018
Toevoegen aan winkelmandje

Beschrijving

Beschrijving

Description: The 4D11 monoclonal antibody reacts with Ly-49G2 (also known as LGL-1). The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate recognition domains, which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor. Ly-49G2 is an inhibitory receptor expressed on subsets of natural killer (NK) cells, NK-T cells (in all strains tested), and on a population of memory CD8+ T cells (in C57BL/6 mice). The 4D11 antibody has been reported to cross react with the Ly-49AB6, Ly-49ABALB, and Ly-49T129/J inhibitory receptors and Ly-49LCBA/J activatingreceptors. The proportion of NK-T cells expressing Ly-49A and Ly-49G2 is higher in the thymus than in liver, and there is evidence that down-regulation of Ly-49 receptor expression is necessary for normal NK-T-cell development to occur.Applications Reported: This eBio4D11 (4D11) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio4D11 (4D11) antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor™ 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specificatie

Specificatie

Ly-49G2
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q60654
Killer cell lectin-like receptor 7, Lymphocyte antigen 49g, Ly-49g, T-cell surface glycoprotein Ly-49G, Klra7, Ly-49g, Ly49-g, Ly49g, Ly49g4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
eBio4D11 (4D11)
PerCP-eFluor 710
Klra7
Liquid
Klra7
IgG2a, kappa, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
16638
Video's

For Research Use Only.