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Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE-Cyanine5, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
€ 184.00 - € 396.00
Specifications
Antigen | Ly-6A/E (Sca-1) |
---|---|
Clone | D7 |
Concentration | 0.2 mg/mL |
Applications | Flow Cytometry |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15370540
|
Affymetrix eBioscience
15-5981-82 |
100 μg |
€ 396.00
100µg |
In Stock Log in to see stock. |
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15360540
|
Affymetrix eBioscience
15-5981-81 |
50 μg |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset. Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family. It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice. Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC. Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen. Sca-1 may be involved in the regulation of B and T cell activation.Specifications
Ly-6A/E (Sca-1) | |
0.2 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
P05533, Q64253 | |
Ly6a, Ly6e | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
Ly6e |
D7 | |
Flow Cytometry | |
PE-Cyanine5 | |
Rat | |
Mouse | |
110454, 17069 | |
IgG2a κ | |
Affinity chromatography | |
Antibody |
For Research Use Only.