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Invitrogen™ Ly-6K Monoclonal Antibody (B33fc8k), PE, eBioscience™
Rat Monoclonal Antibody
Brand: Invitrogen™ 12-5929-82
This item is not returnable.
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Description
Description: This B33fc8k monoclonal antibody reacts with mouse Ly-6k, a member of the Ly6/uPAR superfamily. Ly-6k is a GPI linked protein predominantly found on the cell surface. One study suggests expression may also be non-membranous. Expression is found on B cells and reported to be on plasma cells as well as cells in the testis. Using the B33fc8k monoclonal antibody, expression is on a B lymphocyte cell subset in the spleen that correlates to transitional cells (CD23-IgM-). In humans Ly-6k has been found associated with carcinomas of the head and testis. Applications Reported: This B33fc8k antibody has been reported for use in flow cytometric analysis. Applications Tested: This B33fc8k antibody has been tested by flow cytometric analyis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.
Required for sperm migration into the oviduct and male fertility by controlling binding of sperm to zona pellucida. May play a role in cell growth (PubMed:18089789). [UniProt].
Specifications
| Ly-6K | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| Q9CWP4 | |
| LY6K | |
| Affinity chromatography | |
| RUO | |
| 76486 | |
| 4°C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Flow Cytometry | |
| B33fc8k | |
| PE | |
| LY6K | |
| 2410015A16Rik; 3110035B01Rik; cancer/testis antigen 97; CO16; CT97; FLJ35226; HSJ001348; Ly6k; ly-6K; lymphocyte antigen 6 complex, locus K; lymphocyte antigen 6 complex, locus K-like; lymphocyte antigen 6K; mLy-6K; up-regulated in lung cancer 10; URLC10 | |
| Rat | |
| 100 μg | |
| Primary | |
| Mouse | |
| Antibody | |
| IgG2a κ |
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