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  4. Invitrogen™ Human MAP4K1 (HPK1), GST Tag Recombinant Protein

Invitrogen™ Human MAP4K1 (HPK1), GST Tag Recombinant Protein

Product Code. 15462870
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Quantity:
1 mg
10 μg
100 μg
Unit Size:
100µg
10µg
1mg
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Product Code. 15462870

Brand: Invitrogen™ PV6355

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Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant human MAP4K1 (1-346) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Validated in LanthaScreen™ Kinase Binding Assay Protocols available for simple use and optimized performance Multiple pack sizes available for convenient use Related Product:LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant, Full Length The gene accession number for MAP4K1 (HPK1) is NP_009112.1.

MAP4K1 (also known as HPK1) or Hematopoietic progenitor kinase 1 is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family. MAP4K1 is a tissue-specific upstream activator of the MEKK/JNK/SAPK signaling pathway. MAP4K1 diminishes T cell receptor (TCR) signaling activity and T cell proliferation by phosphorylating the adaptor protein SLP-76. The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yeast STE20 implicated in the mitogen-activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q92918
Concentration See Label
For Use With (Application) Kinase Assay
Formulation 50mM tris HCl with 0.25mM DTT, 0.1mM EDTA, 10mM glutathione, 0.1mM PMSF, 25% glycerol, 150mM NaCl and no preservative; pH 7.5
Gene ID (Entrez) 11184
Molecular Weight (g/mol) 65 kDa
Name Human MAP4K1 (HPK1), GST Tag
pH Range 7.5
Purification Method Purified
Quantity 10 μg
Storage Requirements -80°C, Avoid Freeze/Thaw Cycles
Regulatory Status RUO
Gene Alias EC 2.7.11.1; Hematopoietic progenitor kinase; hematopoietic progenitor kinase 1; HPK; HPK1; MAP4K1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; mHPK1; mitogen activated protein kinase kinase kinase kinase 1; mitogen-activated protein kinase kinase kinase kinase 1
Common Name HPK1
Gene Symbol MAP4K1
Protein Length 1-346
Product Type Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag GST-tag
Sequence 1-346
Content And Storage -80°C, Avoid Freeze/Thaw Cycles
Assay Entry Biochemical binding/kinetic
Kinase Group Serine/Threonine Kinase
Target Entry MAP4K1 (HPK1)
Expression System Baculovirus
Protein Family Kinases & Inhibitors
Protein Form Recombinant, Full Length
Form Liquid
Protein Subtype Serine/Threonine Kinases
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