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  5. Invitrogen™ Mouse MCP-1 ELISA Kit

Invitrogen™ Mouse MCP-1 ELISA Kit

Product Code. 15531967
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Quantity:
10 x 96 Tests
96 Tests
Unit Size:
96 tests
Pack of 10
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Product Code. 15531967

Brand: Invitrogen™ BMS6005

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527.44 EUR valid until 2025-12-31
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Description

ELISA

The Mouse Monocyte Chemoattractant Protein 1(Ms MCP-1) ELISA quantitates Ms MCP-1 in mouse serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms MCP-1. Principle of the method The Mouse MCP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
TRUSTED_SUSTAINABILITY

Specifications

Assay Range 15.6 to 1,000 pg/mL
Assay Sensitivity 2.2 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Serum, Supernatant
Target Species Mouse
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 20296
Intraassay CV 6.2
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Serum 50 μL, Supernatant 50 μL
Synonym Monocyte chemoattractant protein 1, MCAF, HC11
Target CCL2 (MCP-1)
Test Time 1 hrs 20 mins
Total Assay Time 3 hrs 10 mins
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