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Molecular Probes™ Membrane Permeability Dead Cell Apoptosis Kit with PO-PRO™-1 and 7-Aminoactinomycin D, for flow cytometry
Description
This flow cytometry product detects apoptosis on the basis of changes that occur in the permeability of cell membranes using PO-PRO™-1 and 7-aminoactinomycin D (7-AAD) nucleic acid stains. After staining a cell population with PO-PRO™-1 dye and 7-AAD, apoptotic cells show violet fluorescence, dead cells show violet and red fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished by a flow cytometer that uses both a violet laser and the 488 nm line of an argon-ion laser for excitation. Each kit contains sufficient reagents for approximately 200 flow cytometry tests.
View a selection guide for all apoptosis assays for flow cytometry.
View a selection guide for all apoptosis assays for flow cytometry.
Specifications
Specifications
| Quantity | 1 kit |
| Format | Tube, Slide |
| Product Type | Dead Cell Apoptosis Kit |
| Content And Storage | Contains 1 vial of PO-PRO™-1 dye (500 μL), and 1 vial of 7-AAD (7-aminoactinomycin D, 200 μL). Store in refrigerator (2–8°C) and protect from light. |
| Flow Cytometer Laser Lines | 405, 488 |
| Excitation/Emission | PO-PRO™-1: 434⁄456, 7-AAD: 546⁄647 |
| No. of Reactions | 200 |
| Shipping Condition | Room Temperature |
| Product Line | Vybrant |
| For Use With (Application) | Flow Cytometry |
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For Research Use Only. Not for use in diagnostic procedures.
Product Title
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