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  6. Invitrogen™ MembranePro™ Functional Protein Support Kit

Invitrogen™ MembranePro™ Functional Protein Support Kit

Product Code. 17183029
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Sufficient For:
10 Reactions
Unit Size:
10 reactions
This item is not returnable. View return policy
Regulatory: Restrictions may apply to the purchase of this product and we may need to request additional documentation from you

Product Code. 17183029

Brand: Invitrogen™ A11668

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This item is not returnable. View return policy
Regulatory: Restrictions may apply to the purchase of this product and we may need to request additional documentation from you

Description

For customers studying GPCRs and other membrane proteins, MembranePro™ is the ready-to-use system that delivers enriched, functional membrane proteins efficiently and reliably.

Benefits of MembranePro™ include:

  • Proteins are displayed on human (or other mammalian) cell membranes
  • Cellular quality control and mammalian posttranslational processing helps produce functional protein
  • Proteins bud off as lipoparticles and are easily collected from the culture medium
  • Lipoparticles are enriched with expressed receptors

MembranePro™ workflow is less labor intensive in comparison to traditional production of membrane fractions. Briefly, the MembranePro™ protocol allows you to:

  1. Clone your gene of interest using a TOPO™ vector
  2. Transfect with Lipofectamine™ 2000 and MembranePro™ Reagent into 293FT cells
  3. Harvest lipoparticles 48h post-transfection, concentrate with MembranePro™ Precipitation Mix
  4. Store lipoparticles at -80 C or proceed to downstream assays

MembranePro™ products are offered in two configurations – as an expression kit (TOPO™ vector, Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent, 293FT cells) and as a support kit (Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent). The expression kit is only available in a 10 reaction size; the support kit is available in 10, 60 and 600 reaction sizes.

TRUSTED_SUSTAINABILITY

Specifications

Type MembranePro Functional Protein Support Kit
Sufficient For 10 Reactions
Delivery Type Virus-Like Particles (VLPs)
Quantity 10 reactions kit
Content And Storage •1 x 75 ml MembranePro™ Precipitation Mix (Store at room temperature)
•1 x 0.3 mg (1 mg⁄ml) MembranePro™ Reagent (Store at -20°C)
•3 x 0.75 ml Lipofectamine™ 2000 (Store at +4°C)
•Manual
Selection Agent (Eukaryotic) None
Expression Mechanism Cell-Based Expression
High-throughput Compatibility High-throughput Compatible
Expression System Mammalian
Expressed Protein Type Membrane Protein
Product Line MembranePro™
For Use With (Application) Membrane Protein Expression
Promoter EF-1α
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For Research Use Only. Not for use in diagnostic procedures.

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