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MHC class II (I-A/I-E) Antibody (M5/114.15.2), Alexa Fluor™ 750, Novus Biologicals™

Product Code. 18206131 Shop All Bio Techne Products
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Product Code. 18206131 Supplier Novus Biologicals Supplier No. NBP143312AF750

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Rat Monoclonal Antibody

MHC class II (I-A/I-E) Monoclonal specifically detects MHC class II (I-A/I-E) in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.
TRUSTED_SUSTAINABILITY

Specifications

Antigen MHC class II (I-A/I-E)
Applications Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Classification Monoclonal
Clone M5/114.15.2
Conjugate Alexa Fluor 750
Dilution Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Formulation 50 mM sodium borate with 0.05% sodium azide
Gene Alias class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM
Gene Symbols H2-Ea-ps
Host Species Rat
Immunogen The immunogen for this antibody was MHC Class II.
Purification Method Protein A or G purified
Quantity 0.1 mL
Research Discipline Adaptive Immunity, Asthma, B Cell Development and Differentiation Markers, Cell Biology, Diabetes Research, Immune System Diseases, Immunology, Major Histocompatibility Complexes, Transcription Factors and Regulators
Primary or Secondary Primary
Gene ID (Entrez) 100504404
Test Specificity The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
Target Species Human, Mouse
Content And Storage Store at 4°C in the dark.
Isotype IgG2b κ
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