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MHC Class II (I-Ek) Mouse anti-Mouse, Rat, Clone: 14-4-4S, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5980-85

Additional Details : Weight : 0.01000kg

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Product Code. 15227657

  • € 243.00 / 500µg


Estimated Shipment
22-06-2021
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Description

Description

Description: The 14-4-4S monoclonal antibody reacts with the mouse and rat major histocompatibility complex class II, I-E subregion-encoded glycoprotein in mice strains of H-2^k, H-2^d, H-2^p and H-2^r. H-2^b, H-2^f and H-2^s haplotypes do not express the I-E antigen. MHC class II is present on B cells, monocytes, macrophages, and dendritic cells. The 14-4-4S mAb has been reported to block antigen presentation in in vitro assays. This antibody cross-reacts with rat. Applications Reported: 14-4-4S has been reported for use in flow cytometric analysis, immunoprecipitation, complement-dependent cytotoxity and immunohistochemical staining of frozen sections (Biotinylated 14-4-4S, cat. 13-5980, is recommended for IHC). Applications Tested: The 14-4-4S antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Specifications

Specifications

MHC class II (I-Ek)
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P14439
AI323765, E-alpha-f, H-2Ea, I-Ealpha, Ia-3, Ia3, MGC117888
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse, Rat
Flow Cytometry
14-4-4S
Unconjugated
H2-Ea-ps
Liquid
H2-Ea-ps
IgG2a, kappa
500 μg
4° C
Primary
100504404
Videos
SDS
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For Research Use Only.