missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Immunoassay Reagents and Kits
  4. Life Science Research ELISA Kits
  5. 15934214

Invitrogen™ Human MICB ELISA Kit

Product Code. 15934214
Click to view available options
Quantity:
96 Tests
Unit Size:
Pack of 96
This item is not returnable. View return policy

Product Code. 15934214

Brand: Invitrogen™ BMS2303

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

ELISA

The Human MICB ELISA quantitates Hu MICB in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MICB.

Principle of the method

The Human MICB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MICB encodes the highly polymorphic MHC (HLA) class I chain-related gene B. The protein product is expressed on the cell surface. It is thought that MICB functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. MICB is broadly recognized by NK cells and T cells with NKG2D receptor on their surface. The complex NKG2D-MICB results in MICB expressing cytolytic T cells and NK cells against epithelial tumor cells.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q29980
Assay Range 3 to 200 pg/mL
Assay Sensitivity 1.24 pg/ml
Conjugate HRP
Product Type ELISA
Sample Type Cell Lysates, Plasma, Serum, Supernatant
Target Species Human
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 4277
Gene Symbol MICB
Interassay CV 7.1%
Intraassay CV 4.6
Kit Contents Pre-coated 96 well plate, Standard (2 vials), Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target MICB
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs
Show More Show Less
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.