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Invitrogen™ Di-2-ANEPEQ (JPW 1114)
Molecules that fluoresce in response to electrical potential changes in their environment
Brand: Invitrogen™ D6923
Additional Details : Weight : 0.00525kg
Description
- Fast-response probes that operate by means of change in their electronic structure, and consequently their fluorescence properties, in response to change in surrounding electric field
- Optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells, and intact brains
- Magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show 2-10% fluorescence change per 100mV
- Display potential-dependent shift in their excitation spectra, thus permitting quantitation of membrane potential using excitation ratio measurements
- Cationic molecule; exhibits very low internalization and good signal:noise ratio and is useful for neural network analysis
- Ex/Em maxima bound to model phospholipid membranes are approximately 465/635nm (but spectral properties are highly dependent on environment)
- Nonfluorescent until bound to membranes
- Cationic molecule, soluble in water
- Detects submillisecond membrane potential changes
- Dye is introduced into cells by direct addition of stock solution to cell culture medium, by using by retrograde labeling
- Plasma membrane of cell has transmembrane potential of approximately -70mV (negative inside) as a consequence of K+, Na+, and Cl- concentration gradients that are maintained by active transport processes
- Potentiometric probes offer indirect method of detecting the translocation of these ions
- Increases and decreases in membrane potential-referred to as membrane hyperpolarization and depolarization, respectively-play central role in many physiological processes, including nerve-impulse propagation, muscle contraction, cell signaling and ion-channel gating
Cell Analysis, Cell Metabolism, Cell Structure, Cell Viability, Proliferation and Function, Membranes (General) and Lipids
Order Info
Shipping Condition: Room temperature
Specifications
Infrared | |
Fluorescent | |
ANEP Dye | |
Cell Membranes & Lipids |
Store at room temperature and protect from light. | |
Fluorescence Microscope, Microplate Reader | |
Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.