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Invitrogen™ EnzChek™ Ultra Amylase Assay Kit

Product Code. 10093552
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Quantity:
1 Kit
Unit Size:
Each
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Product Code. 10093552

Brand: Invitrogen™ E33651

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This item is not returnable. View return policy

Description

Measures amylase activity or screens amylase inhibitors in a high-throughput format

The EnzChek Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek kit contains a starch derivative—the DQ™ starch substrate—that is labeled with BODIPY FL dye to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.

  • Label (Ex/Em): BODIPY FL conjugate (approx. 502/512nm)
  • Kit contains lyophilized substrate, 10X reaction buffer, substrate solvent, a fluorescent standard, and a detailed protocol
  • Sufficient reagents are supplied for 500 assays (using a 100μL assay volume in a 96-well microplate assay format)

Find Fluorescent Substrates for Other Glycosidases
In addition to the EnzChek Ultra Amylase Assay Kit, we offer kits and substrates to measure xylanase, lysozyme, β-galactosidase, and more.

Cell Analysis, Cellular Imaging, Enzyme and Protein Activity Assays, Glycosidase, Neuraminidase, and Related Enzyme Activity, High-Content Screening (HCS), Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store at room temperature and protect from light.
Detection Method Fluorescence Intensity
For Use With (Application) Ultra Amylase Assay
For Use With (Equipment) Fluorescence Microplate Reader
Product Type EnzChek Assay Kit
Product Line EnzChek
Quantity 1 Kit
Shipping Condition Room Temperature
Substrate Properties Molecular Substrate
Substrate Type Amylase Substrate
Target Enzyme Amylase
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For Research Use Only. Not for use in diagnostic procedures.

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