Invitrogen™ EnzChek™ Protease Assay Kit, green fluorescence, 100-1000 assays

The EnzChek Protease Assay Kit, green fluorescence, is a fast, simple and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with fluorescence microplate reader, is proportional to protease activity. This EnzChek Protease Assay Kit contains a casein derivative that has been extensively labeled with the pH-insensitive, green-fluorescent BODIPY FL dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY FL dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The green-fluorescent BODIPY FL dye has excitation and emission spectra similar to those of fluorescein.

• Generalized mechanism allows for measurement of a variety of proteases
• Can be used to continuously measure protease kinetics
• Excitation/emission of 505/513nm allows use of standard fluorescein (FITC) filter settings

Cell Analysis, Cell Culture, Cell Viability, Proliferation and Function, Differentiation and Development, Enzyme and Protein Activity Assays, Protease/Peptidase Activity, Proteins, Expression, Isolation and Analysis, Stem Cell Analysis, Stem Cell Differentiation, Stem Cell Protein Analysis, Stem Cell Research