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GFP Polyclonal Antibody, Alexa Fluor™ 555, Invitrogen™
Rabbit Polyclonal Antibody
Brand: Invitrogen A31851
Additional Details : Weight : 0.00450kg
Description
At the time of preparation, the products are certified to be free of unconjugated dyes and are tested in a cytological experiment to ensure low nonspecific staining. Several Alexa Fluor™ dye-conjugates made from the rabbit anti-GFP IgG fraction are also available. The Alexa Fluor™ dyes provide for extraordinarily bright antibody conjugates. The approximate fluorescence excitation (Ex) and emission maxima for this Alexa Fluor™ 555 conjugate are 555 nm and 565 nm, respectively. These anti-GFP antibody conjugates are suited for detection of native GFP, GFP variants, and most GFP-fusion proteins by western blot analysis and immunocytochemistry. For initial experiments, we recommend trying dilutions ranging from 1:200 to 1:2,000 with our fluorophore-labeled antibodies, a final concentration of 1-10 μg/mL should be satisfactory for most immunocytochemical applications. If using the rabbit anti-GFP antibody-Alexa Fluor™ conjugates in western blotting, dilute the conjugate 1:2,000 in PBS to obtain a final antibody concentration of 1 μg/mL. HeLa and U2-OS cells were tested with this antibody in immunocytochemistry, but not with paraffin-embedded sections. It is a good practice to centrifuge the protein conjugate solutions briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step eliminates any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
GFP Polyclonal antibody specifically detects GFP in Tag samples. It is validated for ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot.Order Info
Shipping Condition: Wet Ice
Specifications
GFP | |
Polyclonal | |
Alexa Fluor 555 | |
GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP | |
The GFP was isolated directly from the jellyfish Aequorea victoria. | |
100 μL | |
Primary | |
Antibody | |
IgG |
ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot | |
2 mg/mL | |
PBS with 5mM sodium azide; pH 7.5 | |
Rabbit | |
Ion-exchange chromatography | |
RUO | |
4° C, store in dark | |
Liquid | |
Tag |
For Research Use Only. Not for use in diagnostic procedures.