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Description
The OX-33 antibody specifically recognizes a high-molecular-weight form of CD45 found only on B lymphocytes. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
Specifications
| CD45RA | |
| Monoclonal | |
| 0.2mg/mL | |
| Ptprc; Lca; Leucocyte common antigen | |
| Mouse | |
| Affinity Purified | |
| RUO | |
| Rat | |
| IgG1 κ |
| Flow Cytometry | |
| OX-33 | |
| Brilliant Violet 421 | |
| Aqueous buffered solution containing ≤0.09% sodium azide. | |
| Leukocyte common antigen purified from rat splenocytes | |
| 50 μg | |
| Primary | |
| Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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