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Proteintech™ Mouse CCL11/Eotaxin ELISA Kit

Product Code. 16841636 Shop All Proteintech Products
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Target Species:
Mouse
Unit Size:
96 tests
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Product Code. Target Species unitSize
16841636 Mouse 96 tests
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Product Code. 16841636 Supplier Proteintech™ Supplier No. KE1004396T

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KE10043 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL11 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL11.

  • The assay recognizes mouse CCL11. An antibody specific for CCL11 has been pre-coated onto the microwells.
  • The CCL11 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CCL11 is added to detect the captured mouse CCL11 protein.
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent.
  • Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
  • CCL11 also named Eotaxin, was initially discovered as an eosinophil-specific chemoattractant. CCL11 plays a central role in both allergic and non-allergic inflammatory reactions by recruiting immune cells such as eosinophils,basophils and Th2 lymphocytes.
  • CCL11 binds to the chemokine receptors CCR2, CCR3 and CCR5, with highest affinity to CCR3.
  • High levels of CCL11 have been described in several chronic inflammatory diseases, such as allergic rhinitis, atopic dermatitis, asthma, gastrointestinal disease and rheumatoid arthritis.

Specifications

Accession Number BC027521
Assay Range 7.8 to 500 pg/mL
Assay Sensitivity 2.9 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Mouse
Gene ID (Entrez) 20292
Host Species Mouse
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P48298
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Product Identifier
  • 96TEST Mouse CCL11/Eotaxin ELISA Kit
Signal Word
  • Warning
Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
  • MIXTURE LIST-Contain: Sulphuric Acid, Tetramethylbenzidine
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