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  5. Invitrogen™ Mouse GM-CSF ProQuantum Immunoassay Kit

Invitrogen™ Mouse GM-CSF ProQuantum Immunoassay Kit

Product Code. 17144303
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Quantity:
96 Tests
Unit Size:
Pack of 96
This item is not returnable. View return policy

Product Code. 17144303

Brand: Invitrogen™ A46735

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This item is not returnable. View return policy

Description

ELISA

Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.

  • High sensitivity-detect low levels of protein with greater sensitivity than traditional methods
  • Broad dynamic range-≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
  • Small sample consumption-use 2–5 μL of sample (compared to 75 μL for triplicate wells with other methods)
  • Fast, easy workflow-2 hours from sample to answer, with no wash steps
  • No proprietary instrument to purchase-runs on any real-time PCR instrument

The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number colony stimulating factor 2; colony stimulating factor 2 (granulocyte-macrophage); colony-stimulating factor; CSF; CSF2; Csfgm; cytokine; GMCSF; Gm-CSf; granulocyte macrophage colony stimulating factor; granulocyte macrophage-colony stimulating factor; granulocyte-macrophage colony stimulating factor 2; granulocyte-macrophage colony-stimulating factor; MGC131935; MGC138897; MGC151255; MGC151257; MGI-IGM; M-GM-CSF; Molgramostin; put. GM-CSF; sargramostim
Assay Range 0.32 to 25,000 pg/mL
Assay Sensitivity <0.3 pg/mL
Conjugate FAM™
Product Type ProQuantum
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) qPCR Instruments
Gene ID (Entrez) 12981
Gene Symbol CSF2
Kit Contents Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Storage Requirements -20°C
Target GM-CSF
Test Time 30 mins
Total Assay Time 2 hrs
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