Invitrogen™ eBioscience™ Mouse IgE ELISA Ready-SET-Go!™ Kit
For quantitative detection of mouse IgE.
Merk: Invitrogen™ 88-50460-86
Overige gegevens : Gewicht : 0.47500kg
BeschrijvingIgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.
|Colorimetric Microplate Reader|
|Plasma, Serum, Supernatants|
|Plasma (10μL), Serum (10μL), Supernatants (50μL)|
|24 hr 30 min|
|Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)|
|10 x 96 tests|
|50μL (prediluted 1:10)|
|1 hr 45 min|
For Research Use Only