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  5. Invitrogen™ Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 780, eBioscience™

Invitrogen™ Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 780, eBioscience™

Product Code. 16391914
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Quantity:
100 μg
Unit Size:
100µg
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16391914 100 μg 100µg
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Product Code. 16391914 Supplier Invitrogen™ Supplier No. 78472482

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Description

Mouse Monoclonal Isotype Control

This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies. Applications Reported: This mouse IgG2a isotype control has been reported for use in flow cytometric analysis. Applications Tested: This eBM2a antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells. This should be used at the same concentration as experimental antibody. Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497). Light sensitivity: This tandem dye is sensitive to photo-induced o...

Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Mouse IgG2a kappa
Applications Control, Flow Cytometry
Classification Monoclonal
Clone eBM2a
Concentration 0.2 mg/mL
Conjugate Super Bright 780
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Not Applicable
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Isotype Control
Form Liquid
Isotype IgG2a κ
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