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Invitrogen™ Mouse IL-12 Platinum ELISA Kit

For the quantitative detection of mouse IL-12.

Merk:  Invitrogen™ BMS616TEN

Overige gegevens : Gewicht : 0.23750kg

 Bekijk meerdere types van dit product via de

Produktcode. 15542047

  • € 2280.00 / 10 stuks


Geschatte verzenddatum
bij leverancier 30-04-2019
Toevoegen aan winkelmandje

Inclusief: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-12
Biotin-Conjugate anti-mouse IL-12 monoclonal antibody
Streptavidin-HRP
Mouse IL-12 Standard lyophilized, 4ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Beschrijving

Beschrijving

Interleukin-12 (IL-12) is a heterodimeric 70 kDa cytokine composed of two covalently linked, glycosylated chains with molecular weights of 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. Studies have established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. IL-12 p70 is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. Human and mouse IL-12 p40 share about 70% amino acid sequence homology.
Specificatie

Specificatie

P43431, P43432
Biotin
16159, 16160
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
10 x 96 tests
4.0 pg/mL
50μL
Mouse
1 hr 20 min
Mouse
CMLF, NKSF, Interleukin 12
5%
31.3-2,000 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA
RUO
Serum, Supernatants
Serum (50μL), Supernatants (50μL)
2-8°C
3 hr 10 min
Il12a, Il12b
10%
HRP
Video's

For Research Use Only