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  5. Invitrogen™ Mouse LAP/TGFb ProQuantum Immunoassay Kit

Invitrogen™ Mouse LAP/TGFb ProQuantum Immunoassay Kit

Product Code. 17124303
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Quantity:
5 x 96 Tests
96 Tests
Unit Size:
Each
Pack of 96
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Product Code. 17124303

Brand: Invitrogen™ A46342

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This item is not returnable. View return policy

Description

ELISA

Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.

  • High sensitivity-detect low levels of protein with greater sensitivity than traditional methods
  • Broad dynamic range-≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
  • Small sample consumption-use 2–5 μL of sample (compared to 75 μL for triplicate wells with other methods)
  • Fast, easy workflow-2 hours from sample to answer, with no wash steps
  • No proprietary instrument to purchase-runs on any real-time PCR instrument

TGF beta-1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its prodomain. Activation of TGF-b1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF-beta, an aminoterminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number CED; DPD1; LAP; Latency-associated peptide; prepro-transforming growth factor beta-1; regulatory protein; TGFB; TGFB1; Tgfb-1; TGFbeta; TGF-beta 1; TGFbeta1; TGF-beta1; TGF-beta-1; transforming growth factor beta 1; transforming growth factor beta-1; Transforming growth factor beta-1 proprotein; transforming growth factor, beta 1; transforming growth factor-beta 1
Assay Range 0.032 to 2500 ng/mL
Assay Sensitivity <0.03 ng/mL
Conjugate FAM™
Product Type ProQuantum
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) qPCR Instruments
Gene ID (Entrez) 21803
Gene Symbol TGFB1
Kit Contents Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Storage Requirements -20°C
Target LAP
Test Time 30 mins
Total Assay Time 2 hrs
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