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Proteintech™ Mouse/Rat IGF1 ELISA Kit
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Target Species:
Mouse, Rat
Unit Size:
96 tests
Description
- The assay recognizes mouse/rat IGF1. An antibody specific for mouse/rat IGF1 has been pre-coated onto the microwells. The mouse/rat IGF1 protein in samples is captured by the coated antibody after incubation.
- Following extensive washing, another antibody of biotinylated specific for mouse/rat IGF1 is added to detect the captured mouse/rat IGF1 protein.
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
- Insulin-like-growth factor 1 (IGF1), a 70 amino-acid peptide hormone is the principal mediator of biochemical effects of growth hormone (GH). IGF1 is an important growth factor in the regulation of cell proliferation and differentiation.
- IGF1 is largely synthesized in the liver (75%) and, to a lesser extent, in peripheral tissues. IGF1 have been shown to play an essential role in preventing the formation of fatty liver. IGF1 is a potent mitogen and is inhibited by IGF-binding protein-3 (IGFBP3). High serum IGF1 and low IGFBP3 are associated with increased risk of several carcinomas.
- Mice lacking IGF1 exhibit generalized organ hypoplasia including underdevelopment of the central nervous system and developmental defects in bone, muscle and reproductive systems.
Specifications
Specifications
| Accession Number | BC012409 |
| Assay Range | 15.6 to 1000 pg/mL |
| Assay Sensitivity | 1.3 pg/mL |
| Certifications/Compliance | ISO 13485 |
| Detection Method | Sandwich ELISA, Double Antibody |
| Immunoassay Kit Format | Coated ELISA Kit |
| Sample Type | Plasma, Serum |
| Substrate | DAB |
| Target Species | Mouse, Rat |
| Gene ID (Entrez) | 16000 |
| Show More |
Safety and Handling
Product Identifier
- 96TEST Mouse CXCL2 ELISA Kit
- Warning
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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