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Invitrogen™ Mouse Semaphorin 4D/CD100 ELISA Kit

Product Code. 17738083
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Quantity:
10 x 96 Tests
Unit Size:
Pack of 10
This item is not returnable. View return policy

Product Code. 17738083

Brand: Invitrogen™ EM68RBX10

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This item is not returnable. View return policy

Description

ELISA

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number O09126
Assay Range 23 to 5,000 pg/mL
Assay Sensitivity 23 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 20354
Gene Symbol Sema4d
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target CD100 (SEMA4D)
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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