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Invitrogen™ Mouse TNF alpha ELISA Kits

Product Code. 10657534
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Quantity:
5 Plate Kit
Unit Size:
1 set
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Product Code. 10657534

Brand: Invitrogen™ EMTNFA5

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Description

Measure levels of mouse tumor necrosis factor alpha (TNFa) in mouse serum and cell culture supernatant samples using 96-well plates.

Assay TNFα (mouse tumor necrosis factor alpha) levels in mouse serum and culture supernatant samples with our Thermo Scientific™ Mouse TNF alpha ELISA Kits.

These mouse TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched TNFα antibody pairs and TNFα standard only. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant mouse TNFα (mouse tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96 well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 50 to 2450pg/mL

  • Sensitivity: < 9pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNα or human TNFα. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFα in culture supernatants

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

TRUSTED_SUSTAINABILITY

Specifications

Assay Range 50 to 2450pg/mL
Assay Sensitivity <9pg/mL
Description Mouse TNF alpha ELISA Kit
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Sample Type Cell Culture Supernatant, Serum
Specificity No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNa or human TNFa. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFa in culture supernatants
Sufficient For 5 x 96 microplate wells
Kit Contents Streptavidin-HRP Concentrate, 250 μL, TMB Substrate Solution, 5 x 13mL, Stop Solution, 55mL, Standard Diluent, 55mL, Lyophilized Recombinant Mouse TNF alpha Standard, 5 vials, HRP Dilution Buffer, 70mL, Biotinylated Antibody Reagent, 30mL, Anti-Mouse TNF alpha Precoated 96-well Strip Plate, 5 ea, Adhesive Plate Covers, 30 ea, 30X Wash Buffer, 200mL
Measurement Absorbance at 450nm minus absorbance at 550nm
Precision Intra-assay CV <10%; inter-assay CV <10%
Quantity 5 Plate Kit
Sample Volume 50 μL
Target Natural and recombinant mouse TNFa (mouse tumor necrosis factor alpha)
Total Assay Time 3 hr.
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