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Thermo Scientific™ Multiskan SkyHigh Microplate Spectrophotometer
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Description
Multiskan SkyHigh Microplate Spectrophotometer is a UV/Vis microplate reader designed to be convenient and easy to use for any photometric absorbtion or turbidimetric research applications and especially DNA, RNA and protein analysis. This microplate reader is ideal for multi-user environments where a variety of endpoint, kinetic and spectral assays are performed. Multiskan SkyHigh is available in different configurations. Touchscreen models offer the flexibility to use the system as stand-alone, or together with Thermo Scientific™ SkanIt Software for PC control. A model with a cuvette reading option is also available. For convenient data handling, Multiskan SkyHigh has an option to access to Thermo Fisher Connect and Microsoft OneDrive cloud-based tools.
- Monochromator-based optical system for free selection of wavelengths from 200 nm to 1000 nm
- Compatible with 6-48, 96- and 384-well microplates or cuvettes with maximum plate height of 19.5 mm including possible lid
- Reads μDrop™ and μDrop Duo Plates for micro-volume DNA/RNA and protein analysis
- Separate optimized measurement modes for absorption and turbidimetric measurements
- Allows kinetic, spectral, and endpoint measurements for a variety of applications
- Fast operation: full spectrum of a well is done in less than 10 seconds; typical DNA spectrum can be measured in less than three seconds and a full 96-well microplate is read with one wavelength in 6 seconds
- Performs onboard shaking and incubation for temperature-critical or cell-based assays
- Touchscreen models have an easy-to-use interface for stand-alone use, and includes ready-made protocols for UV based nucleic acid and protein quantification as well as colorimetric protein quantification
- Thermo Fisher Connect or Microsoft OneDrive cloud-based tools allow you to securely store, access, share, and manage your photometric data remotely (touchscreen models)
- Multiple languages available; choose between English, German, French, Spanish, Italian, Portuguese, Russian, Chinese, and Japanese (touchscreen models)
- Setting up of more demanding assays is easy via the powerful and intuitive SkanIt™ Software
- Multi-instrument software platform compatible with all Thermo Scientific microplate readers
- Open license software for unlimited installation to multiple computers
- Research Edition for life sciences; Drug Discovery Edition with features for compliance with FDA 21 CFR Part 11
- User-interface languages available: English, French, German, Spanish, Portuguese, Italian, Russian, Japanese, and Chinese
- Searchable SkanIt Cloud Library with ready-to-use protocols including calculations and true application example data
- Measurement sessions are easy to setup and navigate
- Intuitive patented Virtual Pipetting Tool assists in defining the plate layout
Specifications
Specifications
| Cuvettes | No |
| Incubator Temperature | Ambient +2°C to 45°C |
| Interface | PC Control |
| Light Source | Xenon Flash Lamp |
| Measurement Speed | 96 wells: 6 sec., 384 wells: 10 sec. |
| Plate Type | 6, 48, 96, and 384-well Plates, μDrop Plates |
| Product Line | Multiskan |
| Reading Options | Top |
| Shaking | Linear |
| Software Type | SkanIt Software, Open License Software, Multi-Instrument Software |
| Show More |
Frequently Asked Questions (FAQs)
The Multiskan SkyHigh Microplate Spectrophotometer supports clear bottom 6- to 48-, 96- and 384-well microplates with and without lids, where the maximum height of the microplate or a combination of the microplate and lid is 19.5 mm. Please refer to the Plate Selection Table for Plate Readers brochure (https://assets.thermofisher.com/TFS-Assets/BID/Flyers/nunc-plate-selection-table-plate-readers-flyer.pdf) for specific plates to use.
You can also see the specific plate types in SkanIt Software v7.
All of the DNA instruments offered by Thermo Fisher Scientific are of excellent quality. To find the right instrument to quantify DNA or RNA in your lab, explore the detailed comparison of UV-Vis spectrophotometers and fluorometers. Things like sensitivity, throughput and budget may be initial considerations when selecting a DNA quantification instrument. You may also consider whether you need target specificity, sample purity information, RNA quality information, or broad dynamic range.
UV and fluorescence technologies work differently to quantify DNA. UV quantification relies on the intrinsic absorptivity of DNA and RNA molecules, while fluorescence quantification uses dyes that specifically bind to your molecule of choice. With UV technology, quantification isn't as sensitive, but it has a broader dynamic range and also gives data about sample purity‒plus it is faster because there is no reagent prep. With fluorescence technology you get higher sensitivity and molecule-specific data, but it has lower dynamic range and reagent prep is required. The technology you need will depend on what features are important for your lab and experimentation.
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