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Invitrogen™ nNOS Monoclonal Antibody (J23), FITC
Mouse Monoclonal Antibody
Brand: Invitrogen™ MA545958
This item is not returnable.
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Description
A 1:1,000 dilution of MA5-45958 was sufficient for detection of NOS1/Nnos in 7.5 μg of Mouse Brain Cerebellum by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 160.97 kDa.
NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
Specifications
| nNOS | |
| Monoclonal | |
| 1 mg/mL | |
| PBS, 9.1mM sodium bicarbonate with 640.91mM DMSO, 136.36mM ethanolamine and no preservative; pH 7.4 | |
| P29475, P29476, Q9Z0J4 | |
| NOS1 | |
| Partial Human Recombinant NOS1 Protein. | |
| 100 μg | |
| Primary | |
| Human, Mouse, Rat | |
| Antibody | |
| IgG2a |
| ELISA, Immunohistochemistry, Western Blot, Immunocytochemistry | |
| J23 | |
| FITC | |
| NOS1 | |
| 2310005C01Rik; bNOS; bNOS1; Brain NOS; Constitutive NOS; IHPS1; NC-NOS; neuronal nitric oxide synthase; neuronal nitric oxide synthase NOS1; neuronal NOS; nitric oxidase synthase; nitric oxide synthase; nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); nitric oxide synthase 1, neuronal; nitric oxide synthase, brain; nNOS; N-NOS; nNOS; NOS type I; NO; NOS; NOS Type 1; NOS type I; Nos1; Nos-1; NOS-I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS | |
| Mouse | |
| Protein A | |
| RUO | |
| 18125, 24598, 4842 | |
| 4°C, store in dark | |
| Liquid |
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