Invitrogen™ Nop25 Polyclonal Antibody

Description

Immunogen sequence: HQEYLKMLAE REEALEEADE LDRLVTAKTE SVQYDHPNHT VTVTTISDLD LSGARLLGLT PPEGGAGDRS EEEASSTEKP TKALPRKSRD PLLSQRISSL TASLHAHSRK KVKRKHPRRA QDSKKPPRAP RTSKAQRRRL TGKAR Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 86%.

Nop25 is a nucleolar protein predominantly involved in the architecture and function of the nucleolus, which is the cellular hub for ribosomal RNA (rRNA) synthesis and ribosome assembly. It is identified as a putative RNA-binding protein and plays a crucial role in rRNA transcription. Nop25 has been characterized through molecular cloning, showing its ability to bind single-stranded nucleic acids directly. Experimental studies, such as those involving mouse embryogenesis, have utilized techniques like RT-PCR to analyze Nop25's gene expression across developmental stages. Moreover, research on the protein's structure has unveiled its nuclear and nucleolar targeting sequences, vital for its localization to the nucleolus. Treatment of cells with RNase A has illustrated the dissociation of Nop25 from the nucleolus, further highlighting its RNA-binding capabilities. This protein's functional importance is underscored by its role in maintaining nucleolar architecture, as demonstrated by experiments where overexpression of Nop25's N-terminal amino acids disrupted nucleolar integrity.