Invitrogen™ Cytokine Rat Magnetic 10-Plex Panel

Description

By measuring ten analytes simultaneously, the Luminex assay panel providse more data from each sample, saving time and cost compared to traditional systems such as ELISA. This assay panel employs magnetic beads, facilitating automation, decreasing hands-on time, and increasing throughput and precision.

The Rat Cytokine Magnetic 10-Plex Panel for the Luminex platform provides reagents for the accurate, reproducible, and sensitive quantitation of rat proteins including: IL-1β, IL-1α, IL-2, IL-4, IL-6, IL-10, IL-12 (p40/p70), GM-CSF, IFN-γ, and TNF-α.

Luminex xMAP™ Technology—Effective and Proven Analytical Tool
The Rat Cytokine Magnetic 10-Plex Panel is based on xMAP™ technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex assays. Magnetic microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. At the completion of the sandwich immunoassay, beads can be read using the Luminex 200™ or FLEXMAP 3D detection systems, instruments based on flow cytometry, two lasers, real-time digital signal processing, and xPONENT™ software to distinguish bead color (analyte) and assay signal strength (PE) fluorescence intensity.

MAGPIX System—Brings the Power of Multiplex to Every Lab
The MAGPIX system may also be used for sample analysis. This instrument utilizes xMAP™ technology and is built on light-emitting diodes (LEDs) for excitation, a CCD camera for bead and analyte detection, and high-speed digital signal processors. The MAGPIX system is more compact and robust providing the most cost effective magnetic bead-based multiplexing tool available. Simple, out of the box set-up allows researchers to rapidly perform singleplex or multiplex assays of up to 50 targets in single biological samples. The MAGPIX system is easy to use—ideal for both new and experienced users alike.

Magnetic Beads Let You Do More with Your Time
Magnetic bead-based assays enable automation, decrease hands-on time, and increase throughput and precision. Using the fast and easy Luminex protocols, you can obtain your results in as little as 3.5 hours. This line of assays was carefully designed and tested for maximum sensitivity, range, and correlation. Using the same components as our polystyrene bead assays allows for performance with identical quality and consistency. Standard curves generated with this assay system extend over several orders of magnitude of reagent concentrations, while the sensitivity and quality of the assays are comparable to ELISAs and our polystyrene bead assays, enabling superior performance. A natural sample test comparing polystyrene and magnetic bead assays shows magnetic and polystyrene beads give equivalent results.

• Accurate, reproducible and sensitive quantitation of multiple proteins
• Fully qualified antibodies permit specificity and sensitivity
• Performs assays and analyzes data typically in less than one day

See product insert for specifications on each marker in the assay. Suitable for use with the Luminex 200, FLEXMAP™ 3D, and MAGPIX™ systems.

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay