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Invitrogen™ Human Insulin ELISA Kit

Product Code. 10039272
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Quantity:
96 Tests
Unit Size:
96 tests
This item is not returnable. View return policy

Product Code. 10039272

Brand: Invitrogen™ KAQ1251

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554.80 EUR valid until 2025-12-31
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This item is not returnable. View return policy

Description

ELISA

The Human Insulin ELISA Kit quantifies human insulin in serum. The assay will recognize both the natural and recombinant insulin. Principle of the method The Human insulin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P01308
Assay Range 5.1 to 250 μlU/mL
Assay Sensitivity 0.17 μIU/mL
Conjugate HRP
Product Type ELISA
Sample Type Serum
Target Species Human
For Use With (Application) Colorimetric Detection
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3630
Gene Symbol IDDM,IDDM1,IDDM2,ILPR,IRDN,MODY10
Incubation Time 0.75 hr.
Interassay CV 8.5%
Intraassay CV 5.4
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Controls
Label or Dye HRP
Marker Gene Insulin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Serum 50 μL
Storage Requirements 2°C to 8°C
Target Insulin
Test Time 1 hrs 20 mins
Total Assay Time 45 mins
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Safety and Handling

compliance-icons
Product Identifier
  • Insulin Human ELISA Kit
 Signal Word
  • Danger
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Skin corrosion/irritation Category 1 B
 Hazard Statement
  • H314-Causes severe skin burns and eye damage.
  • H318-Causes serious eye damage.
 Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P363-Wash contaminated clothing before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Hydrogen chloride
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