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Invitrogen™ Luminex™ Cytokine Human 25-Plex Panel
Description
Includes
Contains premixed antibody coated capture beads, standard, premixed detector antibody, diluents, SAV concentrate, buffers, wash solution, filter plate, complete protocol and lot-specific technical data sheet.
By measuring 25 analytes simultaneously, the assay panel provides more data from each sample, saving costs and time. This assay may be run alone or in combination with other select Luminex singleplex kits. Suitable for use with Luminex 100™/200 and FLEXMAP 3D™ systems.
Comprehensive human analyte panel
The Human Cytokine 25-Plex Panel for the Luminex platform provides reagents for the accurate, reproducible, and sensitive quantitation of human proteins including: GM-CSF, Eotaxin, IFN-α, IP-10, IFN-γ, MCP-1, IL-1β, MIG, IL-1RA, MIP-1α, IL-2, MIP-1β, IL-2R, RANTES, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40/p70), IL-13, IL-15, IL-17, and TNF-α Luminex xMAP™ technology—effective and proven analytical tool
The Human Cytokine 25-Plex Panel is based on xMAP technology. The use of a suspension bead–based technology enables the multiplexing capabilities of the Luminex assays. Polystyrene microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. At the completion of the sandwich immunoassay, beads can be read using the Luminex 100/200 and FLEXMAP 3D™ detection systems, instruments based on flow cytometry, two lasers, real-time digital signal processing, and xPONENT™ software to distinguish bead color (analyte) and assay signal strength (PE) fluorescence intensity.
More validation for confidence in your results
The Human Cytokine 25-Plex Panel has been validated using the same rigorous criterion that distinguishes Luminex assays. Each kit supplied with a product insert that outlines the specifications for each marker in the assay. Standard curves are generated for each lot of product to assure quantitation consistency.
- Superior performance: accurate, reproducible, and sensitive quantitation of multiple proteins
- High quality: fully qualified antibodies permit excellent specificity and sensitivity
- Fast and easy protocols: perform your multiplex assay and analyze your data typically in less than one day
Cell Analysis, Immunoassays, Ready-To-Use Immunoassay
Specifications
Specifications
| Quantity | 100 Assays |
| Bead Type | Polystyrene |
| Assay Sensitivity | See Product Insert |
| For Use With (Application) | Fluorescent Detection |
| For Use With (Equipment) | Luminex Instruments |
| Gene ID (Entrez) | 1437, 3438, 3458, 3552, 3553, 3558, 3562, 3565, 3567, 3569, 3574, 3576, 3586, 3592, 3596, 3600, 3605, 3627, 4283, 6347, 6348, 6351, 6352, 6356, 7124 |
| Protein Subtype | Eotaxin, GM-CSF, IFN-α, IFN-γ, IL-1β, IL-10, IL-12 (p40/p70), IL-13, IL-15, IL-17, IL-1RA, IL-2, IL-2R, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, MCP-1, MIG, MIP-1α, MIP-1β, RANTES, TNF-α |
| Sample Type | Cell Culture Supernatant, Plasma, Serum |
| Kit Contents | Buffers, Capture antibody coated beads, Complete protocol, Diluents, Filter plate, Lot specific technical data sheet, Premixed detector antibody, SAV concentrate, Standard, Wash solution |
For Research Use Only. Not for use in diagnostic procedures.
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