Macherey-Nagel™ NucleoBond™ Finalizer Large for desalting and concentration of plasmid DNA

The NucleoBond Finalizers are designed for quick concentration and desalination of plasmid and cosmid DNA eluates that are obtained by anion-exchange chromatographic DNA purification with NucleoBond columns. The sample is precipitated with isopropanol and loaded onto a NucleoBond Finalizer membrane by means of a syringe. After an ethanolic washing step and drying of the membrane the pure DNA can be eluted with low salt buffer for further use. The NucleoBond Finalizer technology replaces the tedious and time-consuming centrifugation step which hitherto follows every isopropanol precipitation of diluted DNA samples. Furthermore problems like lost DNA pellets or incomplete solubilisation of hardly visible precipitates can easily be circumvented. The pure concentrated and almost salt free DNA can be used directly for all common down-stream applications like transfection, in vitro transcription, automated or manual sequencing, cloning, hybridisation, and PCR*.

• Elimination of centrifugation steps for precipitation, time saving from >1hr to only 5min
• Two sizes available, to be combined with Midi and Maxi preparations, higher flexibility
• Highly concentrated plasmid DNA depending on elution volume
• No loss of DNA pellets or imcomplete solubilisation of hardly visible precipitates Quick concentration and desalination of plasmid and cosmid DNA elutes obtained by anion-exchange chromatography. DNA suitable for e.g., transfection, in vitro transcription, automated or manual sequencing, cloning, hybridisation and PCR*.