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  5. Macherey-Nagel™ NucleoSpin™ DNA Insect, Mini kit for DNA from insects

Macherey-Nagel™ NucleoSpin™ DNA Insect, Mini kit for DNA from insects

Product Code. 15533626
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No. of Reactions:
10
50
Unit Size:
10 reactions
50 reactions
This item is not returnable. View return policy

Product Code. 15533626

Brand: Macherey-Nagel™ 740470.50

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This item is not returnable. View return policy

Description

Combines enzymatic lysis and mechanical disruption of cell walls for efficient isolation of genomic DNA from a wide variety of insects and crustacean samples (fresh, frozen, dried or ethanol preserved). Complete kit includes NucleoSpin™ Bead Tubes.

Isolation of genomic DNA from insects and crustaceans

  • Fast and convenient procedure combining enzymatic lysis and bead disruption
  • Suitable for any insect or crustacean (e.g., fruit fly, cricket, mealworm, midge larvae, mosquito)
  • High quality DNA from fresh, frozen, dried or ethanol preserved specimen samples
  • Bead Tubes included for difficult-to-lyse insects (due to exoskeletons and reinforced cell walls)
  • Efficient lysis – Compatible with the most common disruption devices
  • Typical downstream applications: multiplex PCR, Southern blotting, enzymatic reactions

Specifications

Content And Storage Room temperature (18 - 25 °C)
Format Mini Spin Column
Isolation Technology Silica MembraneTechnology and Mechanical (Bead Tube) Disruption
Sample Type Insect or Crustacean Specimens (Fresh, Frozen, Dried or Ethanol Preserved)
Efficiency Binding capacity: 60 μg
Elution Volume Elution volume: 25 - 200 μL
For Use With (Equipment) Disruption device (e.g. Vortex-Genie 2 with MN Bead Tube Holder), Centrifuge, Vortex mixer
For Use With (Application) Isolation of genomic DNA from insects and crustaceans
Includes NucleoSpin™ DNA Insect Columns, NucleoSpin™ Bead Tubes Type D, Collection Tubes (2 mL), Buffers
No. of Reactions 50
Purity A260/A280: 1.7 - 1.9
Sample Volume <40 mg fresh, frozen, dried or ethanol preserved insect or crustacean samples
Size Fragment size: 200 bp - approx. 50 kbp
Test Time 35 min/6 prep
Yield <25 μg
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Safety and Handling

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Product Identifier
  • NucleoSpin DNA Insect
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 2
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H317-May cause an allergic skin reaction.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H336-May cause drowsiness or dizziness.
  • H411-Toxic to aquatic life with long lasting effects.
  • H412-Harmful to aquatic life with long lasting effects.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
 Supplemental information
  • MIXTURE LIST-Contains : 1-dodecylpyridiniumchloride, 2-propanol
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