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Invitrogen™ Osteopontin (OPN/SPP1) Mouse ELISA Kit
Sandwich ELISA Kit
€ 626.00 - € 2635.00
Specifications
Accession Number | P10923 |
---|---|
Assay Sensitivity | 4 pg/mL |
Assay | Solid-phase sandwich ELISA |
Conjugate | Biotin |
Product Type | ELISA Kit |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15435333
|
Invitrogen™
EMSPP1 |
96 Tests |
€ 626.00
1 set |
Estimated Shipment: 13-05-2024
Log in to see stock. |
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17145779
|
Invitrogen™
EMSPP1X5 |
5 x 96 Tests |
€ 2635.00
Each |
Estimated Shipment: 03-06-2024
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Description
The Mouse Osteopontin (OPN/SPP1) ELISA quantitates Ms OPN in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms OPN. Principle of the method The Mouse OPN solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.Specifications
P10923 | |
Solid-phase sandwich ELISA | |
ELISA Kit | |
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF. | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
20750 | |
<10% | |
RUO | |
2°C to 8°C | |
1 hr. 20 min. |
4 pg/mL | |
Biotin | |
Cytokines & Receptors | |
Mouse | |
Colorimetric Microplate Reader | |
<12% | |
HRP | |
Bone Research | |
Mouse | |
4 hr. 45 min. |