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Invitrogen™ Human Osteopontin/SSP1 ELISA Kit

Product Code. 17748023
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Quantity:
10 x 96 Tests
Unit Size:
Pack of 10
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17748023 10 x 96 Tests Pack of 10
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Product Code. 17748023 Supplier Invitrogen™ Supplier No. EHSPP1X10

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Description

ELISA

Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P10451
Assay Range 74.07 to 18,000 pg/mL
Assay Sensitivity 50 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 6696
Gene Symbol SPP1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Target Osteopontin
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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