Thermo Scientific™ Wilkins-Chalgren Anaerobe Broth (Dehydrated)

Use Anaerobe Blood Broth for the isolation of general anaerobes from clinical samples and for antimicrobial susceptibility testing of aerobic bacteria using Receiver Operating Characteristic (ROC) procedure.

The formulation of Wilkins-Chalgren Anaerobe Broth includes yeast extract to supply vitamins and other growth factors such as purines and pyrimidines that are necessary for good growth of Peptostreptococcus anaerobius and Bacteroides melaninogenicus. Arginine is added to ensure sufficient amino-acid is available for the growth of Eubacterium lentum. Pyruvate is present as an energy source for asaccharolytic cocci such as Veillonella. It also acts similarly to catalase and degrades traces of hydrogen peroxide, which may be produced by the action of molecular oxygen on medium constituents and interfere with the metabolism of anaerobes. Haemin is found to be essential for the growth of Bacteroides species and menadione for Bacteroides melaninogenicus.