missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit
missing translation for 'orderingAttributeHoverText'
Quantity:
20 μg Kit
missing translation for 'unitSize'
10 reactions
Beschreibung
- Used as a Geneticin™ selectable expression plasmid for engineering stable cell lines
- Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
- WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
- TOPO™ cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products
- Neomycin resistance gene for selection of stable cell lines with Geneticin™
- pUC origin for high copy replication and maintenance of the plasmid in E. coli
Spezifikation
Spezifikation
| Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
| Cloning Method | TOPO™-TA |
| Constitutive or Inducible System | Constitutive |
| Content And Storage | Store pcDNA™3.4-TOPO™ vector and all cloning reagents at -20°C. Store One Shot™ TOP10 Chemically Competent E. coli cells at -80°C. |
| Delivery Type | Transfection |
| For Use With (Application) | Protein Production, Constitutive Expression |
| Plasmid | High Copy Plasmid |
| Product Line | TOPO™ |
| Product Type | TA Cloning Kit |
| Promoter | CMV |
| Mehr anzeigen |
For Research Use Only. Not for use in diagnostic procedures.
Berichtigung von Produktinhalten
Bitte geben Sie uns Ihr Feedback zu den Produktinhalten, indem Sie das folgende Formular ausfüllen.
Name des Produkts
Haben Sie Verbesserungsvorschläge?Übermitteln Sie eine inhaltliche Korrektur